expression vector

(noun)

An expression vector, otherwise known as an expression construct, is generally a plasmid that is used to introduce a specific gene into a target cell.

Related Terms

  • plasmid
  • transcription

Examples of expression vector in the following topics:

  • Shuttle Vectors and Expression Vectors

    • An expression vector is generally a plasmid that is used to introduce a specific gene into a target cell.
    • An expression vector, otherwise known as an expression construct, is generally a plasmid that is used to introduce a specific gene into a target cell .
    • The goal of a well-designed expression vector is the production of large amounts of stable messenger RNA, and in extension, proteins.
    • Cloning vectors, which are very similar to expression vectors, involve the same process of introducing a new gene into a plasmid, but the plasmid is then added into bacteria for replication purposes.
    • In general, DNA vectors that are used in many molecular-biology gene-cloning experiments need not result in the expression of a protein.
  • Plasmids as Cloning Vectors

    • Vectors called expression vectors (expression constructs) express the transgene in the target cell, and they generally have a promoter sequence that drives expression of the transgene.
    • The purpose of a vector is to multiply the insert, although expression vectors also drive the translation of the multiplied insert.
    • However, expression vectors have a two expression patterns: constitutive (consistent expression) or inducible (expression only under certain conditions or chemicals).
    • The above conditions are necessary for expression vectors in eukaryotes, not prokaryotes.
    • Protein purification tags: Some expression vectors include proteins or peptide sequences that allows for easier purification of the expressed protein.
  • Vectors for Genomic Cloning and Sequencing

    • The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes.
    • The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the insert in the target cell.
    • Vectors called expression vectors (expression constructs) are specifically for the expression of the transgene in the target cell, and generally have a promoter sequence that drives expression of the transgene.
    • Simpler vectors called transcription vectors are only capable of being transcribed but not translated: they can be replicated in a target cell but not expressed, unlike expression vectors.
    • These plasmid transcription vectors characteristically lack crucial sequences that code for polyadenylation sequences and translation termination sequences in translated mRNAs, making protein expression from transcription vectors impossible.
  • Adding and Subtracting Vectors Using Components

    • Another way of adding vectors is to add the components.
    • Previously, we saw that vectors can be expressed in terms of their horizontal and vertical components .
    • To add vectors, merely express both of them in terms of their horizontal and vertical components and then add the components together.
    • This new line is the resultant vector.
    • Vector Addition Lesson 2 of 2: How to Add Vectors by Components
  • Components of a Vector

    • Vectors are geometric representations of magnitude and direction and can be expressed as arrows in two or three dimensions.
    • All vectors have a length, called the magnitude, which represents some quality of interest so that the vector may be compared to another vector.
    • Vectors, being arrows, also have a direction.
    • For three dimensional vectors, the magnitude component is the same, but the direction component is expressed in terms of $x$, $y$ and $z$.
    • He also uses a demonstration to show the importance of vectors and vector addition.
  • Scalars vs. Vectors

    • Vectors require both a magnitude and a direction.
    • The magnitude of a vector is a number for comparing one vector to another.
    • In the geometric interpretation of a vector the vector is represented by an arrow.
    • Talking about the direction of these quantities has no meaning and so they cannot be expressed as vectors.
    • An example of a vector.
  • Vectors in Three Dimensions

    • A Euclidean vector (sometimes called a geometric or spatial vector, or—as here—simply a vector) is a geometric object that has magnitude (or length) and direction and can be added to other vectors according to vector algebra.
    • Vectors play an important role in physics: velocity and acceleration of a moving object and forces acting on it are all described by vectors.
    • Thus the bound vector represented by $(1,0,0)$ is a vector of unit length pointing from the origin along the positive $x$-axis.
    • The coordinate representation of vectors allows the algebraic features of vectors to be expressed in a convenient numerical fashion.
    • For example, the sum of the vectors $(1,2,3)$ and $(−2,0,4)$ is the vector:
  • Vector-Valued Functions

    • A vector function covers a set of multidimensional vectors at the intersection of the domains of $f$, $g$, and $h$.
    • Also called vector functions, vector valued functions allow you to express the position of a point in multiple dimensions within a single function.
    • These can be expressed in an infinite number of dimensions, but are most often expressed in two or three.
    • The input into a vector valued function can be a vector or a scalar.
    • In Cartesian form with standard unit vectors (i,j,k), a vector valued function can be represented in either of the following ways:
  • Calculus of Vector-Valued Functions

    • A vector function is a function that can behave as a group of individual vectors and can perform differential and integral operations.
    • A vector-valued function, also referred to as a vector function, is a mathematical function of one or more variables whose range is a set of multidimensional vectors or infinite-dimensional vectors.
    • The input of a vector-valued function could be a scalar or a vector.
    • In terms of the standard unit vectors $\mathbf{i}$, $\mathbf{j}$, $\mathbf{k}$ of Cartesian 3-space, these specific type of vector-valued functions are given by expressions such as:
    • Because vector functions behave like individual vectors, you can manipulate them the same way you can a vector.
  • Introduction to Scalars and Vectors

    • Displacement is an example of a vector quantity.
    • In mathematics, physics, and engineering, a vector is a geometric object that has a magnitude (or length) and direction and can be added to other vectors according to vector algebra.
    • It is any quantity that can be expressed by a single number and has a magnitude, but no direction.
    • (A comparison of scalars vs. vectors is shown in . )
    • He also uses a demonstration to show the importance of vectors and vector addition.
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