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Chapter 17

Biotechnology and Genomics

Book Version 32
By Boundless
Boundless Biology
Biology
by Boundless
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Section 1
Biotechnology
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Biotechnology

Relying on the study of DNA, genomics analyzes entire genomes, while biotechnology uses biological agents for technological advancements.

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Basic Techniques to Manipulate Genetic Material (DNA and RNA)

Basic techniques used in genetic material manipulation include extraction, gel electrophoresis, PCR, and blotting methods.

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Molecular and Cellular Cloning

Molecular cloning reproduces the desired regions or fragments of a genome, enabling the manipulation and study of genes.

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Reproductive Cloning

Reproductive cloning, possible through artificially-induced asexual reproduction, is a method used to make a clone of an entire organism.

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Genetic Engineering

In genetic engineering, an organism's genotype is altered using recombinant DNA, created by molecular cloning, to modify an organism's DNA.

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Genetically Modified Organisms (GMOs)

Transgenic modification, adding recombinant DNA to a species, has led to the expression of desirable genes in plants and animals.

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Biotechnology in Medicine

From manipulation of mutant genes to enhanced resistance to disease, biotechnology has allowed advances in medicine.

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Production of Vaccines, Antibiotics, and Hormones

Biotechnological advances in gene manipulation techniques have further resulted in the production of vaccines, antibiotics, and hormones.

Section 2
Mapping Genomes
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Genetic Maps

Genetic maps provide information about which chromosomes contain specific genes and precisely where the genes lie on that chromosome.

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Physical Maps and Integration with Genetic Maps

Physical maps display the physical distance between genes and can be constructed using cytogenetic, radiation hybrid, or sequence mapping.

Section 3
Whole-Genome Sequencing
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Strategies Used in Sequencing Projects

The strategies used for sequencing genomes include the Sanger method, shotgun sequencing, pairwise end, and next-generation sequencing.

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Use of Whole-Genome Sequences of Model Organisms

Sequencing genomes of model organisms allows scientists to study homologous proteins in more complex eukaryotes, such as humans.

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Uses of Genome Sequences

Genome sequences and expression can be analyzed using DNA microarrays, which can contribute to detection of disease and genetic disorders.

Section 4
Applying Genomics
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Predicting Disease Risk at the Individual Level

Genome analysis is used to predict the level of disease risk in healthy individuals.

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Pharmacogenomics, Toxicogenomics, and Metagenomics

The effectiveness and safety of drugs can be determined through pharmacogenomics.

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Genomics and Biofuels

Microbial genomics can be used to create new biofuels.

Section 5
Genomics and Proteomics
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Genomics and Proteomics

Proteomics is the study of the entire set of proteins produced by a cell type in order to understand its structure and function.

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Basic Techniques in Protein Analysis

The basic techniques used to analyze proteins are mass spectrometry, x-ray crystallography, NMR, and protein microarrays.

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Cancer Proteomics

Proteomics, the analysis of proteins, plays a prominent role in the study and treatment of cancer.

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Gene Expression
  • Regulation of Gene Expression
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Chapter 17
Biotechnology and Genomics
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  • Mapping Genomes
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Evolution and the Origin of Species
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