Appendix F2 - Figure 1

Supplement F: Laboratory Guidance

Public Health Guidance for Community-Level Preparedness and Response to Severe Acute Respiratory Syndrome (SARS) Version 2/3

Figure 1 - SARS-CoV Specimen Testing Guidelines: RT-PCR Testing

Text version of Figure 1

For Respiratory specimens; Blood, plasma, or serum; and Stool

Prepare four aliquots¹

1. To test for SARS-CoV
2. For repeat testing
3. For confirmatory testing
4. To test for other agents

Store remaining sample.

Perform SARS RT-PCR

• If Neg result, specimen is Neg for SARS-CoV.²
  • Testing for other pathogens may be indicated.
• If control guidelines are not met, repeat test using new aliquot.
• If Pos result, repeat test using new aliquot.
  • If Neg result, additional testing may be advised.²
  • If Pos result x2, this is presumptive identification of SARS-CoV.
    • Send aliquot to CDC for confirmatory testing. Include copy of data.

Footnotes

1. Preparation of aliquots must be done where there is no risk of contamination.
2. A negative result does not rule out SARS-CoV infection. See Appendix F7 for more information.