Microbiology
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Microbiology

Section 25

Molecular Techniques

Book Version 6
By Boundless
Boundless Microbiology
Microbiology
by Boundless
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10 concepts
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Inactivating and Marking Target Genes with Transposons

Transposons allow genes to be transferred to a host organism's chromosome, interrupting or modifying the function of a gene.

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DNA Sequencing of Insertion Sites

An insertion site is the position at which a transposable genetic element is integrated.

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Northern Blots

Northern blots allow investigators to determine messenger RNA molecular weight and sample content.

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Western Blots

The Western blot technique determines protein molecular weight and measures protein abundance in different samples.

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DNA Mobility Shifts

DNA mobility shift assay is a technique for studying gene regulation and determining protein-DNA interactions.

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Purifying Proteins by Affinity Tag

Protein tags are peptide sequences genetically grafted onto a recombinant protein.

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Primer Extension Analysis

Primer extension is used to map the 5' ends of DNA or RNA fragments.

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DNA Protection Analysis

DNA protection or "footprinting" analysis is a powerful technique for identifying the nucleotides involved in a protein-DNA interaction.

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Whole-Genome DNA-Binding Analysis

Whole-genome DNA-binding analysis is a powerful tool for analyzing epigenetic modifications and DNA sequences bound to regulatory proteins.

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Two-Hybrid Analysis

The two-hybrid method detects the interaction of two proteins by their ability to reconstitute the activity of a split transcription factor.

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