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Amplifying DNA: The Polymerase Chain Reaction

The Steps of PCR

The Steps of PCR

This illustrates a PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers. 1. Denaturing at 96°C. 2. Annealing at 68°C. 3. Elongation at 72°C. The first cycle is complete. The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle.

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    "PCR."
    http://commons.wikimedia.org/wiki/File:PCR.svg Wikimedia CC BY-SA.

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