electrophoresis

(noun)

a method for the separation and analysis of large molecules (such as proteins) by migrating a colloidal solution of them through a gel; gel electrophoresis

Related Terms

Examples of electrophoresis in the following topics:

  • Northern Blots

    • Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence.
    • The term 'Northern blot' actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane.
    • RNA samples are then separated by gel electrophoresis.

  • Immunoblot Procedures

    • Proteins are generally separated by size using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis.
    • In clinical diagnostic settings, immunoelectrophoresis is applied, which involves the electrophoresis of serum or urine followed by immunodiffusion.

  • DNA Sequencing Based on Sanger Dideoxynucleotides

    • Following rounds of template DNA extension from the bound primer, the resulting DNA fragments are heat denatured and separated by size using gel electrophoresis.
    • DNA sequencers carry out capillary electrophoresis for size separation, detection and recording of dye fluorescence, and data output as fluorescent peak trace chromatograms.

  • Genetic Analysis

    • Pulsed field gel electrophoresis (PFGE) is an adaptation of conventional agarose gel electrophoresis that allows extremely large DNA fragments to be resolved (up to megabase size fragments).

  • Western Blots

    • The technique uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide.

  • DNA Protection Analysis

    • The method uses an enzyme, deoxyribonuclease (DNase, for short) to cut the radioactively end-labeled DNA, followed by gel electrophoresis to detect the resulting cleavage pattern.

  • Multiplex and Real-Time PCR

    • It is confirmed by agarose gel electrophoresis for qualitative results.
    • The results from multiplex PCR can be analyzed using gel electrophoresis or using fluorophores for analysis using during the reaction.

  • DNA Mobility Shifts

    • The pattern shown in lane 3 is the one that would result if all the DNA were bound and no dissociation of complex occurred during electrophoresis.