immunoprecipitation

(noun)

A technique in which an antigen is precipitated from a solution by using an antibody, or a particular use of this technique.

Examples of immunoprecipitation in the following topics:

  • Whole-Genome DNA-Binding Analysis

    • The method of chromatin immunoprecipitation, combined with microarrays (ChIP-Chip), is a powerful tool for genome-wide analysis of protein binding.
    • This extract is then subjected to immunoprecipitation (IP) with a specific antibody against the protein of interest.
    • To facilitate immunoprecipitation and subsequent washing, antibodies are usually coupled to either agarose- or magnetic beads via protein A or G.

  • Purifying Proteins by Affinity Tag

    • These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.