dideoxynucleotide

(noun)

any nucleotide formed from a deoxynucleotide by loss of an a second hydroxyl group from the deoxyribose group

Related Terms

Examples of dideoxynucleotide in the following topics:

  • Strategies Used in Sequencing Projects

    • The primer and dNTP are mixed with a small proportion of fluorescently-labeled dideoxynucleotides (ddNTPs).
    • A dideoxynucleotide is similar in structure to a deoxynucleotide, but is missing the 3' hydroxyl group (indicated by the box).
    • When a dideoxynucleotide is incorporated into a DNA strand, DNA synthesis stops.
    • Frederick Sanger's dideoxy chain termination method uses dideoxynucleotides, in which the DNA fragment can be terminated at different points.

  • DNA Sequencing Techniques

    • This sequencing method is based on the use of chain terminators, the dideoxynucleotides (ddNTPs).
    • The dideoxynucleotides, or ddNTPSs, differ from deoxynucleotides by the lack of a free 3' OH group on the five-carbon sugar.
    • By using a predetermined ratio of deoxyribonucleotides to dideoxynucleotides, it is possible to generate DNA fragments of different sizes when replicating DNA in vitro.
    • In Frederick Sanger's dideoxy chain termination method, fluorescent-labeled dideoxynucleotides are used to generate DNA fragments that terminate at each nucleotide along the template strand.