fluorophore

Examples of fluorophore in the following topics:

• Confocal Microscopy

  • Development of modern confocal microscopes has been accelerated by new advances in computer and storage technology, laser systems, detectors, interference filters, and fluorophores for highly specific targets.

• Fluorescence Microscopy

  • The light emitted from the fluorophore is magnified through traditional objectives and ocular lenses.

• Multiplex and Real-Time PCR

  • The results from multiplex PCR can be analyzed using gel electrophoresis or using fluorophores for analysis using during the reaction.
  • It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is often difficult.

• Strategies Used in Sequencing Projects

  • Each ddNTP is labeled with a different color of fluorophore.
  • The different colors of the fluorophore-labeled ddNTPs help identify the ddNTP incorporated at that position.

• The FISH Technique

  • The probe can be tagged directly with fluorophores, or with targets for flourescently labelled antibodies or other substrates.