serial dilution

(noun)

stepwise dilution of a substance in solution

Related Terms

  • dilution

Examples of serial dilution in the following topics:

  • Dilutions of Solutions

    • Serial dilutions involve diluting a stock or standard solution multiple times in a row.
    • For example, a ten-fold serial dilution could result in the following concentrations: 1 M, 0.1 M, 0.01 M, 0.001 M, and so on.
    • Serial dilutions are used to accurately create extremely diluted solutions, as well as solutions for experiments that require a concentration curve with an exponential or logarithmic scale.
    • Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.
    • This video shows how to solve two dilution problems, using the standard dilution formula, M1V1 = M2V2.
  • Viable Cell Counting

    • The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000 etc. ) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated.
    • The initial analysis is done by mixing serial dilutions of the sample in liquid nutrient agar which is then poured into bottles.
    • Calculation of this is a multiple of the counted number of colonies multiplied by the dilution used.
    • Examples of a viable cell count are spread plates from a serial dilution of a liquid culture and pour plates.
    • With a spread plate one makes serial dilutions in liquid media and then spreads a known volume from the last tube in the dilution series.
  • Aseptic Technique, Dilution, Streaking, and Spread Plates

    • A serial dilution is the step-wise dilution of a substance in solution.
    • A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M...
    • Serial dilutions are used to accurately create highly-diluted solutions as well.
    • For a ten-fold dilution on a 1 mL scale, vials are filled with 900 microliters of water or media, and 100 microliters of the stock microbial solution are serially transferred, with thorough mixing after every dilution step.
    • Example of Serial dilution of bacteria in five steps.
  • Calculating Diluted Earnings per Share

    • Diluted earnings per share (EPS) takes the basic EPS formula and accounts for the effect of dilutive shares on earnings.
    • Diluted Earnings Per Share (diluted EPS) is a company's earnings per share (EPS) calculated using fully diluted common shares outstanding (i.e. which includes the impact of instruments such as stock option grants and convertible bonds).
    • Fully diluted common shares consider securities with features that will increase the number of common shares outstanding and reduce (dilute) earnings per share.
    • Diluted earnings per share includes shares of common stock from dilutive securities, such as convertible debt or stock options, in its calculation.
    • Explain why a company would calculate diluted earning per share for its stock
  • Serial and Parallel Processing

    • Serial memory processing compares a memory to a target stimulus, while parallel processing carries out multiple operations simultaneously.
    • Serial memory processing is the act of attending to and processing one item at a time.
    • The serial order of items and the relationships between them can have varying affects on the item's speed and accuracy of recall.
    • Serial memory processing can be either self-terminating or exhaustive.
    • Participants may process some serial memory sets using the self-terminating method and in other cases use the exhaustive method.
  • Earnings per Share

    • Diluted Earnings Per Share (diluted EPS) is a company's earnings per share (EPS) calculated using fully diluted outstanding shares (i.e. including the impact of stock option grants and convertible bonds).
    • To find diluted EPS, basic EPS is first calculated for each of the categories on the income statement.
    • Then each of the dilutive securities are ranked based on their effects, from most dilutive to least dilutive and antidilutive.
    • Calculations of diluted EPS vary.
    • This is adjusted for dilutive shares.
  • Memory Retrieval: Recognition and Recall

    • There are three main types of recall studied in psychology: serial recall, free recall, and cued recall.
    • This is called serial recall and can be used to help cue memories.
    • Serial recall helps a person to remember the order of events in his or her life.
    • When recalling serial items presented as a list (a common occurrence in memory studies), two effects tend to surface: the primacy effect and the recency effect.
    • Like serial recall, free recall is subject to the primacy and recency effects.
  • Seasoned Equity Offering

    • The new shares dilute the value of the existing shares, and this is understandably, typically not looked upon kindly by those who own shares before the dilution.
    • In theory, this dilution should correspond directly to a drop in the share price such that the market capitalization of the company remains the same.
    • The sale of the shares does not benefit the company, and, more importantly, is not dilutive.
    • The dilution of the shares outstanding should theoretically be met with a corresponding drop in share price.
  • The Absent Father and Serial Fatherhood

  • Short-term gains, long-term pain

    • For example, MacMillan Bloedel, one of Canada's largest forest product companies, was labelled a serial forest-clearer and a chronic chlorine user by environmental activists and subsequently lost 5% of its sales almost overnight when it was dropped as a UK supplier by Scott Paper and Kimberley Clark.
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