Microbiology
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Microbiology

Section 12

Tools of Genetic Engineering

Book Version 6
By Boundless
Boundless Microbiology
Microbiology
by Boundless
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7 concepts
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Recombinant DNA Technology

Molecular cloning permits the replication of a specific DNA sequence in a living microorganism.

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Selection

A selectable marker is usually a gene that confers resistance to an antibiotic that would otherwise kill the cells.

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Mutation

Mutations are accidental changes in a genomic sequence of DNA; this includes the DNA sequence of a cell's genome or the DNA or RNA sequence.

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Reproductive Cloning

Reproductive cloning, possible through artificially-induced asexual reproduction, is a method used to make a clone of an entire organism.

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Basic Techniques to Manipulate Genetic Material (DNA and RNA)

Basic techniques used in genetic material manipulation include extraction, gel electrophoresis, PCR, and blotting methods.

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Molecular and Cellular Cloning

Molecular cloning reproduces the desired regions or fragments of a genome, enabling the manipulation and study of genes.

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Plasmids as Cloning Vectors

Plasmids can be used as cloning vectors, allowing the insertion of exogenous DNA into a bacterial target.

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