MacConkey agar

(noun)

A culture medium designed to grow Gram-negative bacteria and differentiate them from lactose fermentation.

Related Terms

  • mannitol

Examples of MacConkey agar in the following topics:

  • Detecting Acid and Gas Production

    • Sorbitol MacConkey agar is a variant of the traditional MacConkey commonly used in the detection of E. coli O157:H7 .
    • Traditionally, MacConkey agar has been used to distinguish those bacteria that ferment lactose from those that do not.
    • In sorbitol MacConkey agar, lactose is replaced by sorbitol.
    • Sorbitol fermenting non pathogenic commensal bacteria from faeces growing on Cefixime Tellurite Sorbitol MacConkey Agar.
  • Cystitis

    • After 24 hours, this inoculated MacConkey agar culture plate cultivated colonial growth of Gram-negative, rod-shaped, and facultatively anaerobic Proteus vulgaris bacteria.
  • Cultivation of Specimen

    • In most cases, specimens are also inoculated into differential media that define such characteristics as fermentation patters (mannitol salt and MacConkey agar) and as reactions in blood (blood agar).
  • Selective and Differential Media

    • Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol.
    • Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila.
    • Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic.
    • MacConkey (MCK), which is differential for lactose fermentationmannitol salt agar (MSA), which is differential for mannitol fermentation.
  • Viable Cell Counting

    • Typical media include Plate count agar for a general count or MacConkey agar to count gram-negative bacteria such as E. coli.
    • The initial analysis is done by mixing serial dilutions of the sample in liquid nutrient agar which is then poured into bottles.
    • The bottles are then sealed and laid on their sides to produce a sloping agar surface.
    • In the pour plate method a diluted bacterial sample is mixed with melted agar and then that mixture is poured into a petri dish.
    • Urine cultured on Oxoid Brilliance UTI Agar plate. 1uL of urine spread onto the agar surface.
Subjects
  • Accounting
  • Algebra
  • Art History
  • Biology
  • Business
  • Calculus
  • Chemistry
  • Communications
  • Economics
  • Finance
  • Management
  • Marketing
  • Microbiology
  • Physics
  • Physiology
  • Political Science
  • Psychology
  • Sociology
  • Statistics
  • U.S. History
  • World History
  • Writing

Except where noted, content and user contributions on this site are licensed under CC BY-SA 4.0 with attribution required.