spectrophotometer

Examples of spectrophotometer in the following topics:

• Measurements of Microbial Mass

  • Spectrophotometers are electrical appliances that can measure turbidity very accurately.
  • Of all the electrical appliances used for counting cells, a spectrophotometer is the cheapest and its operation the fastest and most straightforward.
  • There are spectrophotometers in which several cuvettes can be inserted at one time, reducing work time even more.
  • Additionally, there are spectrophotometers that require extremely small volumes of culture, as little as 1 microliter .
  • This spectrophotometer can measure as little as one microliter of a sample.

• Absorption of Light

  • When studying a photosynthetic organism, scientists can determine the types of pigments present by using a spectrophotometer.
  • Spectrophotometers measure transmitted light and compute its absorption.
  • By extracting pigments from leaves and placing these samples into a spectrophotometer, scientists can identify which wavelengths of light an organism can absorb.

• Complex Ion Equilibria and Solubility

  • The absorbance (A) of the complex is proportional to its concentration (M) and can be measured directly on the spectrophotometer:

• The Importance of Conjugation

  • Only the last group of absorptions are completely displayed because of the 200 nm cut-off characteristic of most spectrophotometers.