pyrosequencing

(noun)

A technique used to sequence DNA using chemiluminescent enzymatic reactions

Related Terms

  • solid
  • gigabase

Examples of pyrosequencing in the following topics:

  • Metagenomics

    • Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get largely unbiased samples of all genes from all the members of the sampled communities.
    • The first metagenomic studies conducted using high-throughput sequencing used massively parallel 454 pyrosequencing.
    • These techniques for sequencing DNA generate shorter fragments than Sanger sequencing; 454 pyrosequencing typically produces ~400 bp reads, Illumina and SOLiD produce 25-75 bp reads.
    • Pyrosequenced metagenomes generate 200–500 megabases, while Illumina platforms generate around 20–50 gigabases.
  • DNA Sequencing Techniques

    • The most popular, widely-used second-generation sequencing method was one called Pyrosequencing.
Subjects
  • Accounting
  • Algebra
  • Art History
  • Biology
  • Business
  • Calculus
  • Chemistry
  • Communications
  • Economics
  • Finance
  • Management
  • Marketing
  • Microbiology
  • Physics
  • Physiology
  • Political Science
  • Psychology
  • Sociology
  • Statistics
  • U.S. History
  • World History
  • Writing

Except where noted, content and user contributions on this site are licensed under CC BY-SA 4.0 with attribution required.