blot

(noun)

method of transferring protein, DNA, or RNA onto a carrier membrane.

Related Terms

  • sodium dodecyl sulfate

Examples of blot in the following topics:

  • Northern Blots

    • Northern blots allow investigators to determine messenger RNA molecular weight and sample content.
    • The term 'Northern blot' actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane.
    • However, the entire process is commonly referred to as Northern blotting.
    • Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern.
    • The major difference is that RNA, rather than DNA, is analyzed in the Northern blot.
  • Western Blots

    • The Western blot technique determines protein molecular weight and measures protein abundance in different samples.
    • Western blot samples can be taken from whole tissue or from cell culture.
    • The name Western blot was given to the technique by W.
    • Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern.
    • Detection of RNA is termed Northern blot.
  • Immunoblot Procedures

    • Immunoblot procedures like protein blotting, or Western blotting, allow individuals to detect specific solubilized proteins from extracts made from cells or tissues, before or after any purification steps.
    • After this, they are transferred to a synthetic membrane via dry, semi-dry, or wet blotting methods.
    • Western blotting is a routine molecular biology method that can be used to semi-quantitatively compare protein levels between extracts.
    • proteins separated by molecular weight and represented by a dark band on a blot.
    • Describe how Western blotting allows individuals to detect specific solubilized proteins from serum or cell or tissue extracts
  • Basic Techniques to Manipulate Genetic Material (DNA and RNA)

    • When DNA is transferred to a nylon membrane, the technique is called Southern blotting; when RNA is transferred to a nylon membrane, it is called northern blotting.
    • Southern blots are used to detect the presence of certain DNA sequences in a given genome, and northern blots are used to detect gene expression.
    • Southern blotting is used to find a particular sequence in a sample of DNA.
    • Northern blotting is similar to Southern blotting, but RNA is run on the gel instead of DNA.
    • In western blotting, proteins are run on a gel and detected using antibodies.
  • DNA Analysis Using Genetic Probes and PCR

    • Example of genetic analysis method using PCR and immobilized oligonucleotide probes: The reverse dot-blot method has several unique properties that are valuable in a diagnostic setting: (1) The typing that results from a single sample can be located on a single strip.
    • This minimizes user labor as well as error potential and allows the use of standardized reagents. (3) Unlike dot-blot/oligonucleotide typing, only the PCR product is labeled, eliminating the potential problem of probes labeled to different specific activities.
    • Spoligotyping, a reverse dot blot assay that detects the presence of a series of unique spacers in the direct repeat (DR) locus, meets the need for a simple and rapid method by which to distinguish M. tuberculosis complex strains.
  • Monoclonal Antibodies

    • The western blot test and immuno dot blot tests detect the protein on a membrane.
  • Basic Techniques in Protein Analysis

    • The western blot, or protein immunoblot, is a technique that combines protein electrophoresis and antibodies to detect proteins in a sample.
    • A western blot is fairly quick and simple compared to the above techniques and, thus, can serve as an assay to validate results from other experiments.
  • The FISH Technique

    • A similar hybridization technique is called a zoo blot.
  • Purifying Proteins by Affinity Tag

    • These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
  • Detecting Acid and Gas Production

    • A throat culture, for example, is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a growing medium; this will allow analysis to screen for harmful microorganisms, such as Streptococcus pyogenes, the causative agent of strep throat.
Subjects
  • Accounting
  • Algebra
  • Art History
  • Biology
  • Business
  • Calculus
  • Chemistry
  • Communications
  • Economics
  • Finance
  • Management
  • Marketing
  • Microbiology
  • Physics
  • Physiology
  • Political Science
  • Psychology
  • Sociology
  • Statistics
  • U.S. History
  • World History
  • Writing

Except where noted, content and user contributions on this site are licensed under CC BY-SA 4.0 with attribution required.