Affinity chromatography

(noun)

Method of separating a biochemical mixture based on highly specific interactions.

Related Terms

Examples of Affinity chromatography in the following topics:

  • Mapping Protein-Protein Interactions

    • Affinity purification of protein complexes coupled to mass spectrometry is carried out as follows: a specific protein (the bait) is manipulated to express an affinity tag.
    • The tag serves as a tool to purify the bait protein and associated proteins by affinity chromatography.

  • Purifying Proteins by Affinity Tag

    • Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique.
    • Chromatography tags are used to alter chromatographic properties of the protein to afford different resolution across a particular separation technique.

  • Electron Affinity

    • Periodic Properties: Part 4, Ionic Charges, Ionization Energy, Electron Affinity - YouTube

  • Metabolomics

    • The term "metabolic profile" was introduced by Horning, et al. in 1971, after they demonstrated that gas chromatography-mass spectrometry (GC-MS; ) could be used to measure compounds present in human urine and tissue extracts.
    • GC-MS is a method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
    • Separation methods: Gas chromatography, especially when interfaced with mass spectrometry (GC-MS), is one of the most widely used and powerful methods.
    • Gas chromatography–mass spectrometry (GC-MS) is a method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

  • Antibodies: Classes and Affinity Maturation

  • Molar Solubility and Relative Solubility

    • A technique called paper chromatography also takes advantage of the relative solubilities of compounds.
    • In paper chromatography, a small amount of the mixture is placed onto the paper approximately 1 cm from the edge.
    • Paper chromatography is an analytical chemistry technique for separating and identifying mixtures.

  • Northern Blots

    • Eukaryotic mRNA can then be isolated through the use of oligo (dT) cellulose chromatography to isolate only those RNAs with a poly(A) tail.

  • Antibody Functions

    • (a) Affinity refers to the strength of single interactions between antigen and antibody, while avidity refers to the strength of all interactions combined.

  • Combustion Analysis

    • Combustion analysis can also be performed using a CHN analyzer, which uses gas chromatography to analyze the combustion products.

  • Crystallographic Analysis

    • .), purification of recombinant proteins (such as chromatography of affinity and gel filtration), enzymatic tests and inhibition measurement (spectrophotometry), crystallization, x-rays crystallography and structural analysis, interactions determination (microcalorimetry, fluorescence, BIAcore), conformational analyses (circular dichroism, ultracentrifugation, light scattering), modifications analysis (mass spectrometry), bioinformatics, and molecular modelisation.