electrophoresis

(noun)

a method for the separation and analysis of large molecules, such as proteins or nucleic acids, by migrating a colloidal solution of them through a gel under the influence of an electric field

Related Terms

  • denaturation
  • polymerase chain reaction

Examples of electrophoresis in the following topics:

  • Basic Techniques to Manipulate Genetic Material (DNA and RNA)

    • Basic techniques used in genetic material manipulation include extraction, gel electrophoresis, PCR, and blotting methods.
    • Gel electrophoresis is a technique used to separate molecules on the basis of size using this charge and may be separated as whole chromosomes or fragments.
    • Gel electrophoresis separates the nucleic acid fragments according to their size.
  • Basic Techniques in Protein Analysis

    • The western blot, or protein immunoblot, is a technique that combines protein electrophoresis and antibodies to detect proteins in a sample.
    • The protein sample is first separated by gel electrophoresis, then transferred to a nitrocellulose or other type of membrane, and finally stained with a primary antibody that specifically binds the protein of interest.
  • Genomics and Proteomics

    • Proteomics is a relatively-recent field; the term was coined in 1994 while the science itself had its origins in electrophoresis techniques of the 1970's and 1980's.
  • DNA Sequencing Techniques

    • After the reaction is over, the reaction is subject to capillary electrophoresis.
    • The DNA is separated by capillary electrophoresis on the basis of size.
  • Genetic Maps

    • RFLPs (sometimes pronounced "rif-lips") are detected when the DNA of an individual is cut with a restriction endonuclease that recognizes specific sequences in the DNA to generate a series of DNA fragments, which are then analyzed by gel electrophoresis.
  • Strategies Used in Sequencing Projects

    • When the reaction mixture is processed by gel electrophoresis after being separated into single strands, the multiple, newly-replicated DNA strands form a ladder due to their differing sizes.
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